Custom Lentivirus Production

Custom Lentivirus Production

Cellomics Technology, LLC provides customers with flexible and best quality custom lentivirus production services, from construct design, to lentivirus production, concentration and titration.

Lentivirus is a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, lentivirus production is laborious and challenging, especially when high titer of lentivirus (>107 transducing unit (TU)/ml) is required. 

We manufacture both Custom and Ready-To-Use lentivirus. Learn more about our Pre-made lentivirus here.   

Our standardized and optimized custom lentivirus production and strict quality control systems enables us to generate high titer of functional lentivirus for our customers. Our titration procedures determine the quantity of functional lentivirus rather than physical lentiviral particles.

Cat. No.  Service Description Vector Titer
(TU/ml)
Volume Turnaround Time
(weeks)
Price
(construct generation not included)

CLVP-106-010ML
Custom Crude Lentivirus Production

N/A

10 ml

2-3

$350


CLVP-107-001ML

Custom Low-Titer of Lentivirus Production
 
     >107

1 ml

 

2-3

 

$1190

 

CLVP
-108-200UL
Custom Medium-Titer of Lentivirus Production

 >108

 200 µl

 2-3

 $1590


CLVP-109-200UL
Custom High-Titer of Lentivirus Production

>109

 200 µl

2-3

$2190


CLVP-110-020UL
Custom Ultra-High-Titer of Lentivirus Production

>1010

 20 µl

2-3

$2490


CLVP-Quota
Custom-Titer and Scale of Lentivirus Production

Custom

Custom

2-3

Complete Quote Form Below

 

Notes:           *    one titer unit of Cellomics Technology, LLC is roughly equivalent to more than 5 units of IFU/ml or TU/ml from our competitor. 
                 
                     **Titer is measured in clone formation unit (CFU) when antibiotics resistant gene is available in construct; titer in transducing units when fluorescence marker is available in construct; genomic qPCR or other assays may be used if none of them is feasible.

              How custom lentivirus production service proceeds:

If lentiviral construct is ready, please provide us:

1. The construct map or sequence. 

2.1~5 µg of your lentiviral construct plasmid DNA or bacterial culture.

We will prepare Endotoxin-free plasmid and transfect our packaging cell line for your lentivirus production. Harvest lentivirus particle followed by concentration and titration.

For 200 ul of lentivirus production,  You will receive eight  25 µl aliquots.

If you need us to generate lentiviral transfer vector for your target gene, please:

1. Choose lentiviral vector format from our vector collection and specify the gene of interest.

2. Please send us 1~5 µg plasmid or bacterial culture for your gene if it is available, otherwise please send us cDNA sequence.

We will subclone the gene of interest or shRNA into chosen lentiviral vector and confirm by sequencing.


To get a custom quote, please complete the Request a Quote Form below. 


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