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Premade Lentivirus → Lentiviral sgRNA CRISPR → Lentiviral NACC2 sgRNA CRISPR/Cas9

Lentiviral NACC2 sgRNA CRISPR/Cas9

NACC2 (NACC Family Member 2) functions as a transcriptional repressor through its association with the NuRD complex. NACC2 recruits the NuRD complex to the promoter of MDM2, a ubiquitin ligase, leading to the repression of MDM2 transcription and subsequent stability of p53/TP53.

This lentivirus expresses human NACC2 sgRNA under the control of the U6 promoter, Cas9 nuclease and puromycin under the control of the EF1a promoter.

Virus: Lentiviral NACC2 sgRNA CRISPR/Cas9
Titer (approx): 1 x 107 TU/ml
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for sgRNA: U6
Promoter for Cas9 nuclease: Ef1a
Species:
 Human
sgRNA target gene:   NACC2
sgRNA Sequence:
 C CTC ATC CGC CGC GAC CTC G
Selection Gene: Puromycin
Biosafety Level: BSL-2
Shipped: Dry ice
Storage: Store at -80°C

User Instructions | Materials Safety Data Sheet | pCRISPR-EF1a-Cas9 vector

 
Catalog Number Product Size Price Qty
GT-10004-50
Lentiviral NACC2 sgRNA CRISPR/Cas9 (1x10^7 TU/ml)
 2x25 ul
Price: $490.00
GT-10004-200
Lentiviral NACC2 sgRNA CRISPR/Cas9 (1x10^7 TU/ml)
 8x25 ul
Price: $1,200.00
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