Lentiviral CYLD sgRNA CRISPR/Cas9

CYLD (CYLD Lysine 63 Deubiquitinase) is a deubiquitinase that specifically cleaves Lys-63-linked polyubiquitin chains, and plays an important role in the regulation of pathways leading to NF-kappa-B activation. Mutations in CYLD gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

This lentivirus expresses human CYLD sgRNA under the control of the U6 promoter, Cas9 nuclease and puromycin under the control of the EF1a promoter.

User Instructions | Materials Safety Data Sheet | pCRISPR-EF1a-Cas9 vector