Lentiviral vector-EF1a, Hyg

Lentiviral vector-EF1a, Hyg contains  Multiple Cloning Sites (MCS) downstream of the EF1 alpha promoter, and hygromycin driven by the PGK promoter as a selection marker. Clone your gene of interest into the MCS, then co-transfect with EZ-LentiPACK Packaging System into packaging cells, such as HEK293T to produce VSV-G pseudotyped lentivirus. Transduced stable cells can be selected by hygromycin.

• High Protein Expression Levels
• Extremely Low recombination rate
• Compatible with 2nd and 3rd generation of packaging systems, ideal with EZ-LentiPACK Packaging System  

Plasmid Map