Custom Crude Lentiviral Vector Production

Cellomics Technology, LLC provides customers with flexible and best quality lentiviral vector production services, from construct design, to lentivral vector production, concentration and tiration.

Lentiviral vector is a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vector is laborious and challenging, especially when high titer of recombinant lentiviral vectors (>10⁷ transducing unit (TU)/ml) is required.

Our standardized and optimized lentiviral vector production lines and strict quality control systems enables us to generate the best quality of recombinant lentivirus for our customers in shortest turnaround time. Our unique lentivirus concentration and purification technologies set strong foundation for us to produce high purity lentivirus. The final lentivirus delivered to customers is preserved in LentiGuard reagent, invented by scientists of Cellomics Technology, LLC, keeping your experiments consistent and reliable all the time.  Finally, all the lentivirus are titrated with functional assay, rather than physical titration assay, such as p24 ELISA, or lentivirus genome copy number in stock determined with qPCR. 

Advantages of our custom lentivirus production service:

1. Functional titration;

2. High purity and low cytotoxicity for in vitro and in vivo; 

3. Preserved in LentiGuard; 

Notes:

1. Lentiviral vector titer by functional unit is determined by Cellomics Technology, LLC, that is equivalent to more than 5 times higher than that determined by physical particle titration, such as p24, conventional qPCR, etc. The functional titer is practical reference to calculate how much lentivirus stock need in target cell transduction experiment.  

2. Our unique concentration and purification technologies using ViroGold greatly enhances lentivirus purity  by getting rid of host proteins from packaging cell lines.  That makes sure the delivered lentivirus in high purity and low cytotoxicity. 

3. All lentiiviruses are preserved in LentiGuard, special reagent invented by scientists of Cellomics Technology, LLC, that will keep lentivirus in high quality and high titer all the time, by protecting lentivirus from damage resulted from freeze-thaw cycles. 

Turn-around Time: 1-2 weeks