EliteTrust Custom Lentivirus Production

Cellomics Technology, LLC provides customers with flexible and best quality lentiviral vector production services, from construct design, to lentivral vector production, concentration and titration

Lentiviral vector is a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vector is laborious and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) is required. 

 We have big collection of premade Ready-To-Use lentiviruses to meet general purposes. Please check out our  Pre-made lentivirus first, to see if we have ready-to-use lentivirus for your purpose.   We also provide custom services to make lentivirus for you if no lentivirus is available to meet you need. 

Our standardized and optimized lentiviral vector production lines and strict quality control systems enables us to generate the best quality of recombinant lentivirus for our customers in shortest turnaround time.

Our unique lentivirus concentration reagent ViroGold and purification technologies set strong foundation for us to produce high purity lentivirus. The final lentivirus delivered to customers is preserved in LentiGuard reagent, invented by scientists of Cellomics Technology, LLC, keeping your experiments consistent and reliable all the time. 

Finally, all the lentivirus are titrated with functional assay, rather than physical titration assay, such as p24 ELISA, or lentivirus genome copy number in stock determined with qPCR. The functional titer is the best reference to calculate how much lentivirus stock need in target cell transduction experiment.  

 

Advantages of our custom lentivirus production service:

1. High Quality Guaranteed with our strict quality control system; 

2. Shortest Turnaround time (3 ~ 5 business days);

3. The Lowest Price (Please let us know if you get lower price anywhere else, we will try our best to match that);

  4. Preserved in LentiGuard, the innovative cryopreservation reagent to keep the virus titer constant; 

  5. You get fully refund for any defect in our products.

 Notes:

1. Lentiviral vector titer by functional unit is determined by Cellomics Technology, LLC, that is equivalent to more than 5 times higher than that determined by physical particle titration, such as p24, conventional qPCR, etc. 
   
2. Our unique concentration and purification technologies using ViroGold greatly enhances lentivirus purity  by getting rid of host proteins from packaging cell lines.  That makes sure the delivered lentivirus in high purity and low cytotoxicity. 
3. All lentiiviruses are preserved in LentiGuard, special reagent invented by scientists of Cellomics Technology, LLC, that will keep lentivirus in high quality and high titer all the time, by protecting lentivirus from damage resulted from freeze-thaw cycles. 
   
4. One unit of lentivirus from Cellomics Technology, LLC is roughly equivalent to more than 5 units of IFU/ml or TU/ml from our competitor.
5.  Turn-around Time: 3-5 days.  
6. For questions and/or order, please email info@cellomicstech.com.

 

Catalog Number	Description	Guaranteed Functional Titer (TU/ml)	Volume	Purity	Price (USD)	Turnaround Time
CLVP-1E8-200	Medium Titer Purified Lentivirus Preparation	>1x108	200 ul (4x50 ul)	Pure*	$360	3-5 days
CLVP-1E8-400	Medium Titer Purified Lentivirus Preparation	>1x108	400 ul (8x50 ul)	Pure*	$510	3-5 days
CLVP-1E9-400	High Titer Purified Lentivirus Preparation	>1x109	400 ul (4x100 ul)	Pure**	$960	3-5 days
CLVP-1E9-800	High Titer Purified Lentivirus Preparation	>1x109	800 ul (8x100 ul)	Pure**	$1490	3-5 days
CLVP-1E10-40	Ultra-High Titer and Ultra-Pure Lentivirus Preparation	>1x1010	40 ul (4x10 ul)	Pure***	$2400	6-10 days
CLVP-Custom-Quote	Custom Scale and Titer Lentivirus Preparation	Custom	Custom	Custom	Quote	Custom
CLVP-EF-DNA	Customer Lentiviral  Transfer Vector Preparation (Optional)	200 ug	200 ul	Endotoxin Free	$60	2 days

 Notes:  * The Crude means that the lentivirus is not purified, however the lentivirus was produced in the medium without fetal bovine serum (FBS). The virus works well with many different cell types.   ** The lentivirus is concentrated and purified after harvested from the medium without FBS, these lentivirus shows no cytotoxicity to most of human or mouse cells.  ***Ultra Pure lentivirus is produced, concentrated and double purified in the medium without FBS, the double purification means that lentivirus goes through two different purification methods to reach ultra high purity, the lentivirus has no noticeable cytotoxicity even to very sensitive cell types like primary T lymphocytes, NK cells or other special cell types.

How custom lentivirus production service proceeds:

If lentiviral construct is ready, please provide us:

1.  You could send us if you have enough plasmid prepared, the plasmid should be endotoxin-free, high quality and concentration no lower than 0.3 µg/µl plasmid, the plasmid quantity has to be enough for packaging corresponding to the production scale.  

We would prefer to prepare the plasmid ourself to make sure the plasmid quality, the plasmid quality is very important factor in lentivirus packaging.

2.  Otherwise, we would need you send us about 5 µg of lentiviral transfer vector plasmid along with construct map or sequence for us to confirm no mutation happened in the plasmid preparation. You need to ship us about 1~5 µg of your lentiviral transfer construct plasmid DNA or bacterial culture.

We will prepare Endotoxin-free plasmid and transfect our packaging cell line for your lentivirus production. Lentiviral particles will be harvested followed by concentration and titration.

 If you need us to generate lentiviral transfer vector for your target gene, please:

1. Choose lentiviral vector format from our vector collection and specify the gene of interest.

2. Please send us 1~5 µg plasmid or bacterial culture for your gene if it is available, otherwise please send us cDNA sequence.

We will subclone the gene of interest or shRNA into the chosen lentiviral vector and confirm its accuracy by sequencing.

Below are two published product applications using our products:

In this study, defect and treated neuronal processor cells (NPCs) were labeled with Cellomics Technology’s GFP/RFP lentivirus and injected into mice with Fragile X syndrome. The demethylation treatment rescued the phenotype. The very efficient labeling of two populations of NPCs allows scientists to track transplanted cells cleanly and test the function of these cells, and identify the rescue.  (Shawn Liu et al, Cell. 2018 Feb 15;172(5):979–992.e6. doi: 10.1016/j.cell.2018.01.012)

The above image shows the co-localization of fortilin and PRX1 in U2OS. The paper using our lentivirus to deliver fortilin-HA and Flag-PRX1 into U2OS, and monoclonal cell clones were generated. The engineered stable cell lines allowed scientists to perform assays including co-immunoprecipitation to identify protein partners of fortilin, generate recombinant human fortilin, etc. The interaction between PRX1 and fortilin was studied, that shading light on how fortilin, a pro-survival molecule, inhibits p53-induced apoptosis.  (Abhijnan Chattopadhyay, et al, Sci Rep. 2016 Jan 4;6:18701. doi: 10.1038/srep18701)

To get a custom quote, please complete the Request a Quote Form below.