Cas 9 lentivirus

CRISPR-Cas9 technology has been widely used for gene editing. To achieve high efficient gene editing, the optimal Cas9 endonuclease expression level is ciritical. Promoter activity varies in different cell types. Cas9 lentiviruses express Cas9 under different constitutive promoters or tet-inducible promoter, providing the flexibility to select a promoter to drive optimal Cas9 expression and achieve high gene editing efficiency. Tet-inducible Cas9 allows to express Cas9 only at certain period of time. 
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