Lentiviral sgRNA CRISPR/Cas9 All-in-One

Premade sgRNA CRISPR/Cas9 lentivirus provides a convenient way for your gene knock-out study, especially gene knock-out in hard-to transfect cells.

CRISPR/Cas9 gene targeting requires a specific single guide RNA (sgRNA). The sgRNA is composed of a  ~20 nucleotide target specific sequence (crRNA) and a Cas9 nuclease-recruiting sequence (tracrRNA). The specific gene targeting can be achieved by simply changing the crRNA sequence in the gRNA. 

Our premade sgRNA CRISPR/Cas9 lentiviruses express sgRNA under the control of a human U6 promoter, Cas9 nuclease and puromycin selection marker under the control of the EF1a promoter. The sgRNA CRISPR/Cas9 cells can be rapidly selected by puromycin. 

The current premade sgRNA CRISPR/Cas9 lentivirus collection includes sgRNA for 606 genes involved in ubiquitination pathway. Those sgRNA lentiviruses can be purchased individually or together as an array.